Winmdi 29 Free Download Software
May 18, 2007 - Software>>>>WinMDI ver 2.9. WinMDI ver 2.9. Size:2027K Language: English Directory:Miscellaneous Software. WinMDI (Windows Multiple Document Interafce for Flow Cytometry). Download Link. (If you want. Melittin Enables Efficient Vesicular Escape and Enhanced Nuclear Access of Nonviral Gene Delivery Vectors* Received for publication, August 29, 2001, and in revised form, October 9, 2001 Published, JBC Papers in Press, October 12, 2001, DOI 10.1074/jbc.M108331200 Manfred Ogris‡, Robert C. Carlisle, Thierry Bettinger§, and Leonard W. Kaznj plennih russkih soldat v chechne.
Title: Flow Cytometry Basic Training 1 Flow Cytometry Basic Training • A Look Inside the Box James MarvinFlow Cytometry Facility Northwestern University 2 (No Transcript) 3 Section I • Background Information on Flow Cytometry 4 The Many Parts of Flow • Experimental design • Sample preparation • Choosing the proper instrument • Setting up the instrument • Collecting the proper data • Interpreting the data • Graphics presentation and publication • Sorting Specific Applications Courses Flow Basics Data Analysis 5 What Is Flow Cytometry? • Flow cells in motion • Cyto cell • Metry measure • Measuring properties of cells while in a fluid stream 6 (No Transcript) 7 Cytometry vs.
Flow Cytometry • Cytometry • Localization of antigen is possible • Poor enumeration of cell subtypes • Limiting number of simultaneous measurements • Flow Cytometry. • Cannot tell you where antigen is.
• Can analyze many cells in a short time frame. • Can look at numerous parameters at once. 8 Uses of Flow Cytometry • It can be used for • Immunophenotyping • DNA cell cycle/tumor ploidy • Membrane potential • Ion flux • Cell viability • Intracellular protein staining • pH changes • Cell tracking and proliferation • Sorting • Redox state • Chromatin structure • Total protein • Lipids • Surface charge • Membrane fusion/runover • Enzyme activity • Oxidative metabolism • Sulfhydryl groups/glutathione 9 Background Info Summary • Flow Cytometry is a quickly expanding technology • Has continually increased in popularity since the mid 1980s. • Gives us the ability to analyze many properties of many cells in very little time 10 Section II • The 4 Main Components of a Flow Cytometer 11 What Happens in a Flow Cytometer? • Cells in suspension flow single file past • a focused laser where they scatter light and emit fluorescence that is filtered and collected • then converted to digitized values that are stored in a file • Which can then be read by specialized software. 12 What Happens in a Flow Cytometer (Simplified) 13 The Fluidics SystemCells in suspension flow single file • You need to have the cells flow one-by-one into the cytometer to do single cell analysis • Accomplished through a pressurized laminar flow system. • The sample is injected into a sheath fluid as it passes through a small orifice (50um-300um) 14 Fluidics Schematic Sample Tube 15 How The Flow Cell Works • The cells from the sample tube are injected into the sheath stream • Flow in a flow cell is laminar.